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於世界生存之証明、中英文之訓練場所。
26 . September
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07 . September

<< I took this photo when I had just arrived in Helsinki-Vantaa Airport.

When I was writing my last blog, I had just become one of the student helpers in Professor Ricky Wong's research laboratory.

Then, time flew......

In late july, I paused my work in the lab and became a checker in the Population Census 2011 launched by the HKSAR Census and Statistics Department.
The census was really interesting and I learnt a lot from that. I had tried to overcome my fear of talking to strangers in this period of time. Although I am still a little bit shy, I got improvement after the doing survey with more than 50 families.

After the census ended, I returned to the lab and continued my study on MicroRNA in NPC.
I really enjoyed working with all the members in the lab who were very kind and patient.
I only got some trends of the amount of MicroRNAs as result in my presentation, but I treasure everything that I had experienced in the lab. I learnt protein assay, western blot, Reverse transcription, Real-time PCR and also how to organise my own logbook.
For these, I sincerely thank Professor Wong for giving me this precious opportunity to work in his lab, Ivy for teaching me all the protocols and giving suggestion on my exchange in Finland, and also all the lab members for being so friendly to me and teaching me lots of survival skills in the lab. 

As you can see from the very first beginning of this blog, yes I am now in Finland~ I am going to stay in Jyväskylä for the whole academic year with my schoolmate Paddy.
Actually, Paddy and I stayed in Helsinki for two days before going to Jyväskylä. Helsinki is such a beautiful place that I wanted to live in Helsinki for longer period of time.

As for Jyväskylä, Mm...... It is also a good city as there are several beautiful lakes near the University. However, I don't get a good arrangement for my apartment, that's why I felt depressed when I moved in.

Well, the world is not perfect any way.
Come what may, I have to solve the problems when they come to me.
By the way, hope I do survive before I fix everything.

Cheer Up and wish me good luck!!!

PR
01 . July


打從我第一天正式踏入lab,細老細Patrick就不斷說要幫我找個研究題目,讓我在這段日子裡得到實戰經驗和成功感,不只局限於學習技巧做伴讀書僮

然後到了昨天,他終於找到了。

我的題目是研究某幾條NPC Cell line,從而在分析NPC和MicroRNA之間的關係。
雖然這只是個小Project,Patrick也要我有心理準備,這些實驗不易做。
由於是RNA相關的研究,大部份時間會用到Real-time PCR,這類實驗本身難度不算高,但對預防Nuclease污染的要求卻非常嚴格,比Tissue culture(TC)的還要麻煩。
TC的實驗只要噴噴酒精就可以了,但酒精對RNase[1] 無效,一切都要靠實驗人員自己小心行事。
這對異常粗心大意的我是一大挑戰……(扶額)

當Ivy終於把log book[2] 交給我時,我知道……這個暑假將會過得極之充實。(YAY)
幸好我不用養寵物Cell,不然我星期六日也要「上班」呢……|||||


[1] RNase:Nuclease的一種。這類Enzyme可以分解RNA,在一般空氣中也存在。(口水有不少,所以做實驗的時候要盡量少說話)
[2] log book:Lab裡的每個人都有一本,紀錄每天實驗流程、結果和注意事項。Project完結的時候要上繳老細,留給師弟師妹作參考之用。

28 . June

Lumix DMC-FZ18,有後期微調Contrast和Brightness。

這個星期應該都在Lab裡度過。
尤如上班的生活,每天朝十晚六。

腦袋累挎玩不到人狼,所以我在家裡的娛樂變成玩弄父上的Prosumer。
在此感謝百變小希Magic Car教我基本理論,讓我下決心好好學習使用這部相機~!


P.S. 有關剛剛過去的週日機場遊記嘛……我有時間和精神的時候會補回的。(扶額)

23 . June


One word to summarize my first day in the research lab: EXHAUSTING.

Standing beside the bench for at least 7 hours broke my legs and the huge influex of information nearly blew up my brain.
I was so tired that I got into bed at 9 that night. (normally I sleep at 3 in the morning)
Actually I thought I would have slept on sofa right after going back home if my mum didn't throw me into the bath... (My mum: DON'T touch anything at home if you return from the biology lab!)

I had written more than 6 pages of notes during the day. There were so many rules and instructions to memorize, especially for the tubes and pipetmant tips.
Our lab has 3 sets of tools for different purpose: general bench use, tissue culture and RNA research. All of us need to choose the correct set of tools carefully, otherwise the objects studied may be contamination and we have to do all the steps again.
Tissue culture (TC) requires the highest level of contaminant-free condition. All steps have to be doen inside the biological safety cabinet and everything must be sterilized before putting into the cabinet. Therefore, what we saw the most on that morning is spraying ethanol, spraying ethanol and spraying ethanol.

Apart from cell culture, Ivy had also introduced several bioassays to Jason and me. We now focus on Western blot first and then move on to the Real-time Polymerase Chain Reaction (PCR). 
Well, although I am not a Hon-Proj student, I still have homework to do as Ivy asked me to read the papers about these bioassays before returning to the lab next monday. 

19 . June

上面是我們生物系其中一個「影棚」,就算是會發螢光的Model都照影可也~(ROCK)


BU生物系學生到三年級的時候,可以選擇寫畢業論文或是做Honour Project。
每位教授都會提供三至四個topics,每個學生最多可以選三個,然後跟JUPAS一樣,以學生的喜好次序、教授的要求和個人意願來「派位」。
所謂的「老細」,就是指負責你Topic的教授。

因為我九月要到芬太家留學一年,回到香港才開始做Honour Project,所以我這個暑假都不用準備做lab。
不過為了熟習實驗室環境,以及攏絡教授的心方便下年選TOPIC(?),考試過後我就向一位研究癌症的教授申請當Student Helper~

好友Grace先前也當過教授的helper,她說那裡的人對student helper挺冷淡,因為Helper不算是教授的正式學生,要是關係太親密的話,怕有人狐假虎威就麻煩了。
話雖如此,其實上星期我到Lab參觀的時候,大家都對我的態度還好,而且教授今年收的學生都是我關係要好的同學,所以這方面情況應該不會太差。(抹汗)

名義上我是Student Helper,實際上我一點Help的能力也沒有,只是來學習實驗室技巧,好讓我三年級的時候不至於雞手鴨腳,以及避免我在芬太家出洋相。(扶額)
上星期教授把今年收的三年級生名單交給我,要我選一個與他一起學習(感覺有點像陪讀書僮)
本來我打算選一位topic與NPC(鼻煙癌)相關的同學,但一看到負責的Postgraduate Mentor是她男友時,我就立即將她閉屏了。(拜託我不要當電燈膽或者捲入任何非必要麻煩之中!)
思考整整一晚,我最後決定當Jason的同伴~
這位男生頭腦思路清晰,上星期present的時候教授讚他一步到位,絕無半點多餘內容。
希望在跟他一起做Lab當書僮的日子裡,我能夠學習到他的思維模式吧~(有時我總覺得自己廢話太多了)

明天就要上第一課,和Jason一起跟Ivy(就是Jason的Mentor)學做Cell Culture。
聽好友兼教授本年正式學生之一的Grace說,Jason要養的是Primary Cell,所以會有點麻煩,但願我不會出亂子吧……(合手)

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ABOUT ME

Llarsa 拿撒
11/13

他稱白榊.舊稱白神/月和
嫌煩兼懶.故保留羅馬拼音

表面看似正常.內裡實為脫線
/百合愛好者.雙性向實踐人士

目前狀態:
頹廢大學生.生物系背德人士
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